28 , 410421 (1985) Acknowledgements The work was supported by the Monash University Ramaciotti Centre for Cryo-Electron Microscopy, the National Health and Medical Research Council of Australia (NHMRC) project grants (1061044, 1065410, 1120919 and 1126857), NHMRC program grant (1055134), Strategic Priority Research Program of the Chinese Academy of Sciences (XDA12020347) and Shanghai Science and Technology Development Fund (15DZ2291600)
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The sections were microwaved in citrate buffer (PH = 6) for 10 min, and 3% H 2 O 2 blocked the endogenous peroxidase at room temperature for 20 min